Evaluation of Fibrin Clot Adhesion as a Precursor for New Attachment following Root Conditioning

The objective of the present study was to evaluate the influence of various root conditioning modalities on adsorption and adhesion of blood to scaled and planned root surfaces. This research work was done in the Department of Periodontics, DAV Dental College, Yamunanagar, Haryana, India (Year 2006-2009). The SEM analysis was done in ...SEM & TEM centre, Department of Radiology, AIIMS, New Delhi (India) A total of 24 specimens were obtained from fresh extracted human teeth which were divided into 4 groups comprising of 6 specimens in each group. The root conditioning groups included 3 experimental – Citric acid, EDTA (Ethylene diamine tetra acetic acid), Tetracycline hydrochloride and 1 PBS (Phosphate buffered saline) as control group. The root surfaces were planned and specimen blocks (4x4x1mm) were obtained. They were subject to various conditioning agents and then exposed to fresh blood which was allowed to clot. These specimens were then rinsed and subjected to SEM analysis. The results showed that Citric acid treated planned root specimens presented a dense blood cells and fibrin attachment, while tetracycline treated showed moderate and EDTA treated showed scarce attachment of blood clot elements. In contrast, untreated planned dentin exhibited smear layer. The adhesion of fibrin network and blood cell attachment was best following application of Citric acid followed by moderate attachment seen in Tetracycline.